The Interaction of Endochitinase, a Synthetic Peptide ESF12 and Resveratrol in Controlling Fungal Growth in vitro

Ali, G.S., G.E. Harman, and B.I. Reisch. 2000. The interaction of endochitinase, a synthetic peptide ESF12 and resveratrol in controlling fungal growth in vitro. Phytopathology (submitted)

Abstract

The efficacy of two-way combinations of Trichoderma endochitinase (CHIT42), synthetic peptide ESF12 and resveratrol upon the control of growth of Botrytis cinerea and Penicillium digitatum were evaluated in vitro. A colorimetric method for monitoring fungal growth inhibition was compared with the conventional method based on microscopic measurement of spore germination inhibition and germ tube length inhibition. A strong correlation between both methods was found. Subsequent experiments were performed using the colorimetric fungal growth monitoring system. All combinations of CHIT42 + ESF12, CHIT42 + resveratrol, and ESF12 + resveratrol showed additive activity (Interaction Ratios between 0.5 and 1.5) against B. cinerea and P. digitatum. The CHIT42 had EC50 (effective concentration for 50% growth inhibition) values of 60.0 g mL-1 and 1.13 g mL-1 against B. cinerea and P. digitatum, respectively. CHIT42 also caused swelling, hyperbranching and bursting of P. digitatum at concentrations as low as 0.09 g mL-1. The EC50 values of ESF12 were 47.7 M and 37.7 M against B. cinerea and P. digitatum, respectively. Resveratrol had EC50 values of 238.0 M and 453.7 M against B. cinerea and P. digitatum, respectively. No abnormal morphological changes were observed in either of the species when treated with ESF12 or resveratrol.

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